Trevecca’s athletic program is divided into three basic areas: intercollegiate athletics, intramural sports, and a student activities program.

The intercollegiate athletic program, begun at Trevecca in the winter of 1968-69, includes baseball, basketball, cross country/track, soccer, and golf for men and basketball, volleyball, softball, cross country/track, soccer, and golf for women. The intercollegiate athletic program is under the administrative leadership of the director of intercollegiate athletics and the Intercollegiate Athletic Committee (composed of faculty, administrators, alumni, and students). Trevecca became a full member of NCAA Division II in 2014 and is a charter member of the Great Midwest Athletic Conference (2013).

The intramural program is under the administrative supervision of the director of athletics and the immediate sponsorship of the Trevecca Intramural Association. The TIA commissioner is selected each year through an application and interview process and serves as an ex-officio member of the ASB executive committee. Sports included in the intramural program are flag football, soccer, basketball, dodgeball, volleyball/sand volleyball, softball, tennis, and ultimate frisbee.

The student activities program at the physical education venues includes the use of the gymnasium (when available), racquetball courts, athletic fields (when available), tennis courts, jogging track, wellness center, and a lighted outdoor basketball and sand volleyball court.